earticle

영어

Aldose reductase catalyzes the conversion of glucose into sorbitol. Inhibiting this enzyme in diabetes mellitus can delay or prevent pathogenic process. Aldose reductase inhibitor was screened from Korean fermented soybean pastes (Doen-jang) and purified via sequential processes of ethanol extraction, HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, and crystallization. Aldose reductase inhibitor was identified as genistein with molecular weight of 270 Da and molecular formula of C15H10O5 based on UV spectrometry, 1H and 13C NMR3, and mass spectrometry. Genistein inhibited aldose reductase of pig lens with IC50 level of 20 uM. Because genistein was effective against aldose reductase of animal source, it may be a potential therapeutic agent for diabetic complications.