earticle

영어

In this study, the crude methanol extract of mulberry leaves was fractionated with chloroform, ethyl acetate, n-butanol, and water, successively. The antioxidant activities of the fractions were examined with the 2-deoxyribose oxidation and linoleic acid peroxidation methods. The ethyl acetate fraction showed the strongest antioxidant activity. From it we isolated chlorogenic acid, caffeic acid, quercetin 3-O-B-D-glucopyranoside, and kaempferol 3-O-B-D-glucopyranoside with preparatory octadecyl silane-high performance liquid chromatography (ODS-HPLC), and identified the compounds by nuclear magnetic resonance (NMR) and fast atom bombardment mass (FAB-MS) analyses. Overall, quercetin 3-O-B-D-glucopyranoside showed the strongest antioxidant activity by both the 2-deoxyribose oxidation and rat liver microsome peroxidation methods