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Research Article

복령 균핵, 균사체 및 자실체의 추출용매별 생리활성 성분 비교

원문정보

Comparison of the biological activity of extracts from the mycelium, sclerotium, and fruiting body of Wolfiporia cocos (F.A. Wolf) Ryvarden & Gilb using different extraction solvents

안기홍, 조재한, 김옥태, 이찬중, 한재구

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

The aim of this study was to investigate the biological activity of extracts obtained from the mycelium, sclerotium, and fruiting body of Wolfiporia cocos using different extraction solvents (hot water, 70% ethanol, and 70% methanol). Among the three developmental stages, the mycelium extracts showed the highest DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, ferric reducing antioxidant power (FRAP), nitrite scavenging activity, and total polyphenolic content. Among the extraction solvents in the context of the W. cocos mycelium, the DPPH radical scavenging activity, FRAP, and total polyphenol content in the hot-water extracts were significantly higher than those in the other extracts. In the case of the sclerotium, the reducing power, nitrite scavenging activity, and total polyphenol content were significantly higher in 70% ethanolic extracts. The fruiting body showed the highest DPPH radical scavenging activity, reducing power, nitrite scavenging activity, and total polyphenol content in the context of hot-water extraction. Moreover, the β-glucan content was significantly higher in the sclerotium versus the mycelium or fruiting body. The total amino acid and total essential amino acid contents were remarkably higher in the mycelium and fruiting body than in the sclerotium; of note, and arginine (Arg) and phenylalanine (Phe) were highly detected among the amino acid components.

목차

ABSTRACT
서 론
재료 및 방법
공시균주 및 접종원 제조
복령 재배
추출용매별 분석용 시료 제조
DPPH 라디컬 소거능 측정(DPPH radical-scavengingactivity)
철 환원 항산화능 측정(FRAP, Ferric-reducing antioxidantpower)
환원력 측정(Reducing power)
아질산염 소거능 측정(Nitrite-scavenging activity)
총 폴리페놀 함량 측정(Total polyphenol contents)
베타글루칸 함량분석(β-glucan contents)
아미노산 분석 시료조제
HPLC에 의한 아미노산 성분 분석
통계처리
결과 및 고찰
추출용매별 복령 균사체, 균핵 및 자실체의 DPPH 라디컬 소거능
추출용매별 복령 균사체, 균핵 및 자실체의 철 환원 항산화능
추출용매별 복령 균사체, 균핵 및 자실체의 환원력
추출용매별 복령 균사체, 균핵 및 자실체의 아질산염 소거능
추출용매별 복령 균사체, 균핵 및 자실체의 총 폴리페놀함량
복령 균사체, 균핵 및 자실체의 베타글루칸 함량
복령 균사체, 균핵 및 자실체의 아미노산 성분 함량
적 요
REFERENCES

저자정보

  • 안기홍 Gi-Hong An. 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과
  • 조재한 Jae-Han Cho. 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과
  • 김옥태 Ok-Tae Kim. 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과
  • 이찬중 Chan-Jung Lee. 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과
  • 한재구 Jae-Gu Han. 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과

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