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논문검색

미생물/발효/생물공학

메밀 속성장 유래 효소활성 우수 저영양성 균주 분리 및 특성

원문정보

Isolation and Characterization of Oligotrophic Strains with High Enzyme Activity from Buckwheat Sokseongjang

이성영, 김지연, 백성열, 여수환, 구본성, 박혜영, 최혜선

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

Bealmijang is a short-term fermented regional product that is prepared with soybean and extra ingredients. In this study, starter strain candidates were screened from Bealmijang for fermented soybean paste products. Twenty one bacterial strains producing extracellular enzymes (amylase, cellulase, protease, xylanase and lipase) were isolated from Bealmijang, buckwheat sokseongjang. The isolates were assessed for fibrinolytic and antibacterial activities, and salt tolerance. Strain HJ18- 4, identified as Bacillus subtilis (AB601598) by biochemical properties (89.6%) and 16S rDNA sequencing (100%), showed the highest enzymatic, fibrinolytic, and antibacterial activities among the isolates. Although the growth of HJ18-4 was inhibited by the increase of NaCl concentration, the growth still exceeded that of B. subtilis KACC 10114 at 5% and 10% NaCl. These results suggest that B. subtilis HJ18-4 is suitable as a starter for soybean paste manufacture.

목차

Abstract
 서론
 재료 및 방법
  시료
  균주의 분리
  세포 외 효소 활성 측정
  혈전 용해능 측정
  항균 활성 측정
  분리 균주의 특성 및 16S rDNA 유전자 염기서열 분석
  분리 우수 균주의 내염성
 결과 및 고찰
  메밀 속성장 유래 세포외효소 분비능 우수 균주의 선발
  유용 균주의 혈전 용해능
  유용 균주의 항균 활성
  유용 균주의 동정
  유용 균주의 내염성
 요약
 감사의 글
 문헌

저자정보

  • 이성영 Sung Young Lee. 농촌진흥청 국립농업과학원 농식품자원부
  • 김지연 Ji-Yeun Kim. 농촌진흥청 국립농업과학원 농식품자원부
  • 백성열 Sung Yeol Baek. 농촌진흥청 국립농업과학원 농식품자원부
  • 여수환 Soo-Hwan Yeo. 농촌진흥청 국립농업과학원 농식품자원부
  • 구본성 Bon Sung Koo. 농촌진흥청 국립농업과학원 농업생명자원부
  • 박혜영 Hye Young Park. 농촌진흥청 국립농업과학원 농식품자원부
  • 최혜선 Hye-Sun Choi. 농촌진흥청 국립농업과학원 농식품자원부

참고문헌

자료제공 : 네이버학술정보

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