원문정보
초록
영어
Bealmijang is a short-term fermented regional product that is prepared with soybean and extra ingredients. In this study, starter strain candidates were screened from Bealmijang for fermented soybean paste products. Twenty one bacterial strains producing extracellular enzymes (amylase, cellulase, protease, xylanase and lipase) were isolated from Bealmijang, buckwheat sokseongjang. The isolates were assessed for fibrinolytic and antibacterial activities, and salt tolerance. Strain HJ18- 4, identified as Bacillus subtilis (AB601598) by biochemical properties (89.6%) and 16S rDNA sequencing (100%), showed the highest enzymatic, fibrinolytic, and antibacterial activities among the isolates. Although the growth of HJ18-4 was inhibited by the increase of NaCl concentration, the growth still exceeded that of B. subtilis KACC 10114 at 5% and 10% NaCl. These results suggest that B. subtilis HJ18-4 is suitable as a starter for soybean paste manufacture.
목차
서론
재료 및 방법
시료
균주의 분리
세포 외 효소 활성 측정
혈전 용해능 측정
항균 활성 측정
분리 균주의 특성 및 16S rDNA 유전자 염기서열 분석
분리 우수 균주의 내염성
결과 및 고찰
메밀 속성장 유래 세포외효소 분비능 우수 균주의 선발
유용 균주의 혈전 용해능
유용 균주의 항균 활성
유용 균주의 동정
유용 균주의 내염성
요약
감사의 글
문헌
