원문정보
초록
영어
Epigallocatechin-3-gallate (EGCG) is a polyphenolic compound frequently found in green tea, and its physiological actions have been extensively investigated. In the present study, changes in chemical stability and cytotoxic properties of EGCG in the presence of different types of antioxidants were investigated. The antioxidants used modulated the chemical stability of EGCG. Superoxide dismutase (SOD) significantly increased EGCG stability; EGCG was less stable in the presence of catalase. Ascorbic acid, N-acetylcysteine (NAC), and glutathione (GSH) stabilized EGCG concentration dependently. The H2O2 level generated from EGCG was decreased by catalase, SOD, and NAC but not by GSH. The cytotoxic effects of EGCG also decreased in the presence of NAC, catalase, and SOD. GSH, however, showed a complicated modulatory pattern according to the EGCG and GSH concentrations, and ascorbic acid rather enhanced EGCG toxicity. The results suggest that certain antioxidants could modulate the cytotoxic properties of EGCG in a cell culture system not only by removing reactive oxygen species but by modulating chemical stability and other factors, which should be considered carefully when studying reactive oxygen species-dependent mechanisms of EGCG.
목차
서론
재료 및 방법
실험재료
세포주 및 세포배양
다양한 항산화제들의 EGCG 화학안정성에 미치는 영향 평가
Hydrogen peroxide(H2O2) 생성량 측정
각종 항산화제들의 EGCG 세포독성에 대한 영향 평가
통계분석
결과 및 고찰
다양한 항산화제들 존재 시의 EGCG 화학안정성 및 H2O2 생성량의 변화
다양한 항산화제 존재 하에 EGCG의 세포독성 변화
요약
문헌