원문정보
Inhibitory Effect of Cacao Bean Husk Extract on Glucosyltransferase from Streptococus mutans B13
초록
영어
The inhibitory effect of cacao bean husk (CBH) extract on glucosyltransferase(GTasc) from Streptococcus mutans B13 was investigated. Water solube extract from CBH showeda sarong inhibitory effect (88-89%) on GTase from Streptococcus mutans Bl3. GTase inhibitors from sequential extraction by hot water or water-methanol had the strongest inhibition. Sources, fermentation, and types of solvents and fumigation processes did not influence the effect. These active compounds proved to be polyphones through acid hydrolytic analysis of the precipitates by ammonium sulfate or ethanol and proteinase K. It was also confirmed by additional column chromatography of Sephadex G-50, Sephadex LH-20 and DEAE-Sephdex A-50.
목차
서론
재료 및 방법
재료
CBH 추출물의 조제
CBH 추출물의 침전 및 가수분해 처리
Glucosyltransferase 저해물질의 분획
GTase의 활성 및 저해율 측정
CBH 추출물의 물리화학적 특성 조사
GTase 활성저해 분획물의 성분분석
결과 및 고찰
CBH 추출물의 GTase 활성 저해효과의 검토
GTase 저해활성의 요인 성분 검토
GTase 활성 저해물질의 분획 및 동정
결론
참고문헌