earticle

영어

Levan oligosaccharides and low molecular weight levan were produced from levan by acid hydrolysis and following column chromatography. Levan hydrolysis was progressed proportionally as increased incubation time. In terms of levan hdrolysis reaction, no differences were found from the sources of levan. Optimum hydrolysis conditions for the formation of levan oligosaccharides were, 0.38 M H2SO4; and incubation at 95oC for 4 min. The purified products were determined as the mixture of oligosaccharides (degree of polymerization (DP) of 3-6). Two of lactic acid-producing bacteria, Lactobacillus plantarum KCTC 3104 and Pediococcus
pentosaceus KCTC 3507, were studied in vitro for their ability to metabolize levan oligosaccharides. Apparently, the growth of both cells were increased by levan oligosaccharide diet, compared with those of levan diets, suggesting that levan oligosaccharides may be beneficial in selectively growth of lactic acid-producing bacteria.

한국어

산가수분해법과 칼럼크로마토그라피법을 이용하여 레반 올리고당과 저분자량 레반을 생산하였다. 레반에 대한 산가수분해반응은 시간의존적으로 비례적으로 진행되었으며, 다른 미생물 유래의 레반을 사용시에도 동일한 결과를 나타내었다. 레반 올리고당의 제조를 위한 최적화된 조건은 5% 레반을 0.38 M 황산, 95oC, 4분간 처리하였을 때 였으며, 최종생산물로 중합도 3-6의 레반 올리고당을 얻었다. 생성된 레반 올리고당을 탄소원으로 이용하여 두 젖산 생성균주(Lactobacillus plantarum KCTC 3104와 Pediococcus pentosaceus KCTC 3507)의 생장배지에 첨가하고 레반 자체를 첨가한 배지와 비교시, 뚜렷한 생육촉진효과와 pH 감소효과가 나타났다.