원문정보
초록
영어
To treat hyperpigmentating diseases such as melasma and lentigines, a number of
melanogenesis inhibitors have been screened for their effectiveness in reducing melanin, but
none are completely satisfactory. There still remains a need for novel skin depigmenting agents.
In this study, melanogenesis inhibitory and free radical scavenging activity of water soluble
extract of Sterculiae Lychnopherae were examined. The water soluble fraction showed
significant inhibition of melanogenesis (25.89%) and exhibited no cytotoxicity at a
concentration of 200 μg/ml. Inhibitory activity on tyrosinase, the key enzyme of melanogenesis
and antioxidant activity were further investigated. Test results indicated that it did not inhibit
mushroom tyrosinase activity. Based on DPPH assay, this fraction showed antioxidant activity
with IC50= 115 μg/ml. To clarify the target of this fraction action in melanogenesis, RT-PCR and
western blotting were performed. This fraction exhibited the inhibitory effect on mRNA, protein
level of MITF, tyrosinase and TRP-1. This study suggests that the water soluble fraction of
Sterculiae Lychnopherae may be considered as a candidate anti-melanogenic agent and it may
be an effective agent in treating hyperpigmentation disorders