원문정보
초록
영어
D- and L-lactic acid are optical isomers, which are formed by distinct stereo-specific NAD-dependent lactate dehydrogenases (LDH) in lactic acid bacteria(LAB). Leuconostoc (Ln.), the major bacterial population in kimchi, has been predicted to synthesize only D-lactic acid from pyruvate, as they harbor a single D-LDH gene but L-LDH gene. D-lactic acid may lead to acidosis in infants and in adults with short bowel syndrome. As one of the major D-lactic
acid producers in kimchi, it is required to switch the major lactate isoform of Ln. citreum from D- to L-. Hence, with the above long-term goal, first, we have determined the profiles of D-/L- lactic acid synthesis of LAB culture broth. Secondary, we cloned ldhL gene from Lactobacillus plantarum and expressed it in Ln. citreum. Finally, the characteristics of the recombinant Ln.
citreum were investigated.