원문정보
초록
영어
The antioxidant activities of the extracts of dangyuja (Citrus grandis Osbeck) leaves were evaluated. The highest phenolic content was obtained from the ethyl acetate fraction (EF) (202.1±0.8 mg GAE/g dried extract) and it exhibited the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. The cytoprotective effects of EF on oxidative
damage induced by tert-butyl hydroperoxide (t-BHP) in a human hepatoma cell line, HepG2 cells, were investigated to understand the intracellular antioxidant mechanisms. Treatment of HepG2 cells with EF prior to oxidative stress was found to inhibit reactive oxygen species (ROS) generation, lipid peroxidation, and DNA damage in a dose-dependent manner. Gas
chromatography-mass spectrometry (GC-MS) studies on EF resulted in tentative identification of 19 compounds representing 94.3% of the total content. Taken together, these results demonstrated that EF has excellent antioxidant activities and thus dangyuja leaves have great potential as a source for natural antioxidant which can be applied in food products.
목차
Introduction
Materials and Methods
Materials
Preparation of extracts
Determination of total phenolics and flavonoids
Gas chromatography
GC-MS
Free radical scavenging activity
Cell culture
Determination of ROS generation
Measurement of lipid peroxidation
Determination of DNA damage (Comet assay)
Cytotoxicity assay
Statistical analysis
Results and Discussion
Total phenolic content and total flavonoid content
DPPH free radical scavenging activity
Effect of EF on t-BHP-induced intracellular ROSgeneration
Compositional analysis of EF
References