원문정보
초록
영어
The unripe fruit of Momordica charantia (MC) has been shown to possess antidiabetic activity. However, the mechanism of its antidiabetic action has not been fully understood. In this study, the effects of the aqueous ethanolic extract of MC (AEE-MC) were evaluated on the apoptosis in pancreatic β-cells treated with a combination of the cytokines, interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ. In MIN6N8 cells, the inhibitory effect of AEE-MC was
significantly observed at 2 to 50 μg/mL: a 26.2 to 55.6% decrease of cytoplasmic DNA fragments quantified by an immunoassay.
The molecular mechanisms, by which AEE-MC inhibited β-cell apoptosis, appeared to involve the inhibition on the expression of p21, Bax, and Bad, the up-regulation of Bcl-2 and Bcl-XL, and the inhibition on the cleavage of caspase-9, -7, and -3 and poly (ADP-ribose) polymerase. This study suggests that MC may inhibit cytokine-induced apoptosis in β-cells and, thus, may contribute via this action to the antidiabetic influence in diabetes.
목차
Introduction
Materials and Methods
Momordica charantia (MC)
Preparation of MC extract
Cell lines and reagents
Exposure to proinflammatory cytokines
Cell survival assay
Measurement of cell death
Preparation of cell lysates and immunoblotting
Statistical analysis
Results and Discussion
Effects of cytokines on cell viability, apoptosis, and proapoptoticproteins in MIN6N8 cells
Effect of MC extracts on cell viability, cytokine-inducedcytotoxicity, and apoptosis in MIN6N8 cells
Effects of AEE-MC on cytokine-mediated activation ofPARP and caspases
Effects of AEE-MC on the expressions of Bcl-2 familyand p21 in cytokine-treated MIN6N8 cells
References