earticle

영어

The present study was conducted to explore the anti-inflammatory action of 2-hydroxyethyl-β-undecenoi (HPS) purified from Cumin (Cuminum cymium L.) seed against periodontitis. From the study in which human leukocyte was employed to detect the inhibiting effects of 5-lipokygenase and cyclooxygenase, enzymes generating carriers of infection like LTB4 and PGs, as well as of collagenase and elastase, organ-destroying enzymes, following conclusions could be drawn: HPS was found to inhibit leukotrien B4 biosynthesis by stimulating more than 97% of human polymorphonuclear leukocyte (PMNL) with addition of 5×10-2 M when IC50 was set at 2.5×10-4 M. Ninety-two percent of enzyme activation turned out to be inhibited when 5×10-2 M was added in a test to prove inhibiting effects of HPS against activation of PMNL 5-lipoxygenase from homogeneous humans and purified 5-lipoxygenase on the market. Besides, IC50 for enzyme activation was valued at 2.5×10-4 M, while the value of IC50 for purified 5-lipoxygenase was 2.5×10-4 M. The IC50 values of COX-activated leukocyte and purified collagenase were 5.1×10-4 M and 2.3×10-4 M, respectively. Moreover, the value of IC50 for activation of leukocyte collagenase was 2×10-3 M, whereas that for purified collagenase was 5×10-2 M. In case of leukocyte elastase, addition of 5×10-2 M inhibited its activation by 66%. In case of purified one, however, activation of enzyme was inhibited by 25% with addition of 5×10-2 M. Furthermore, the IC50 value for activation of leukocyte elastase was revealed to be 7.5×10-3M. From the virulence test with human gingiva cell, it was shown that, on the second day of cultivation, 47.83% of the cell had been activated when HPS was added by 5×10-2 M. Even the addition of HPS by 1×10-2M featured 68.53% of cell activation, suggesting relatively strong toxicity of the substance against gingiva cell.

한국어

본 연구자들은 Cumin(Cuminum cymium L.) seed로부터 정제한 2-hydroxyethyl-β-undecenoi의 항치주염 활성을 사람의 백혈구를 이용하여 염증 매개물질 LTB4 및 PGs의 생성 효소인 5-lipoxygenase와 cyclooxygenase, 및 조직파괴 효소인 collagenase와 elastase의 저해 효과를 규명하여 다음과 같은 결론을 얻었다. HPS의 항치주염 활성을 사람의 백혈구를 이용하여 염증 매개물질 LTB4 및 PGs의 생성 효소인 5-lipoxygenase와 cyclooxygenase 및 조직파괴효소인 collagenase와 elastase의 저해효과를 규명한 결과, HPS은 5×10-2 M 첨가 시 97%의 PMNL로부터 LTB4 생합성이 저해되었으며 이때 IC50 값은 2×10-4 M 이었다. 균질화된 사람의 PMNL 5-lipoxygenase 및 시판 정제 5-lipoxygenase 활성에 대한 HPS의 저해 효과는 5×10-2 M 첨가 시 92%의 효소 활성이 저해되었다. 또한 효소 활성에 대한 IC50 값은 2.5×10-4 M이었으며, 정제 5-lipoxygenase에 대한 IC50 값은 2.3×10-4 M이었다. 또한 백혈구 COX 활성에 대한 IC50 값은 5.13×10-4 M이었고, 정제 COX에 대한 IC50 값은 2.3×10-4 M이었으며, 백혈구 collagenase 활성에 대한 IC50 값은 2×10-3 M이었고 정제 collagenase에 대한 IC50 값은 5×10-2 M 이었다. 백혈구 elastase의 경우 5×10-2 M 첨가 시 66%의 활성이 저해된 반면 정제 elastase의 경우 5×10-2 M 첨가 시 25%의 효소 활성이 저해되었다. 또한 백혈구 elastase 활성에 대한 IC50 값은 7.5×10-3 M이었다. 인체 치은세포에 대한 독성 시험 결과 5×10-2 M 의 HPS 첨가시 세포의 활성은 배양 2일째 47.83%로 나타났으며, 1×10-2 M의 HPS 첨가시에도 68.53%로 나타나 비교적 세포독성이 강한 것으로 나타났다.