earticle

영어

The present study was conducted to examine the antioxidant activities and neuroprotective effects of methanolic extracts from Schizonepeta tenuifolia Briquet (STE). STE (100 ug/mL) showed 43.33 uM of total phenolic content, 64.43% of radical scavenging activity, and 0.157 of reducing power. In addition, the effect of STE on H2O2-induced DNA damage in human leucocytes was evaluated by the comet assay, where STE was a dose dependent inhibitor of DNA damage induced by 200 uM of H2O2. The protective effect of STE against H2O2-induced oxidative damage on PC12 cells was investigated by an 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) reduction assay and lactate dehydrogenase (LDH) release assays. After 2 hr of cell exposure to H2O2 (500 uM), a marked reduction in cell survival was observed. However, this reduction was significantly prevented by 1-50 ug/mL of STE. Therefore, these results suggest that STE could be a new antioxidant candidate against neuronal diseases.