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논문검색

Development of Detection Method for Cyclomaltodextrinase Family Genes using Degenerate PCR Primers

원문정보

Oh, Su-Won, Jang, Myoung-Uoon, Jeong, Chang-Ku, Yuk, Jeong-Bin, Park, Jung-Mi, Park, Kwan-Hwa, Kim, Tae-Jip

한국식품과학회 Food Science and Biotechnology Volume 15 Number 6 2006.12 pp.967-974
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Cyclomaltodextrinases (CDases), maitogenic amylases, and neopullulanases share highly conserved primary structures and similar characteristics, and are thus classified into the same family. BLAST search has showed that a variety of bacterial strains harbor putative CDase family genes with several well-conserved motif amino acid sequences. In this study, four degenerate polymerase chain reaction (PCR) primer sets were designed for the detection of CDase genes, on the basis of their highly conserved amino acid blocks (WYQIFP, DGWRLD, LGSHDT, and KCMVW). The PCR detection conditions were optimized and the detection specificity of each for the primer sets was tested against the genomic DNAs isolated from 23 different Bacillus-associated species. Consequently, all tested primer sets evidenced successful amplification of specific PCR products in length, which share 55-98% amino acid sequence identity with known and putative CDases. The primers developed herein, therefore, can be applied for the easy and efficient detection and isolation of CDase family genes for the modification of functional food carbohydrates.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

키워드

저자정보

  • Oh, Su-Won Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University
  • Jang, Myoung-Uoon Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University
  • Jeong, Chang-Ku Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University
  • Yuk, Jeong-Bin Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University
  • Park, Jung-Mi Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University
  • Park, Kwan-Hwa Center for Agricultural Biomaterials, and Department of Food Science and Biotechnology, School of Agricultural Biotechnology, Seoul National University
  • Kim, Tae-Jip Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University

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