earticle

영어

Effect of Oenanthe javanica ethanol extract (OJE) on nuclear factor-xB (NF-xB)-mediated inflammatory reaction in RAW 264.7 macrophage cells was investigated. The OJE dose-dependently inhibited secretions of tumor necrosis factor-a (TNF-a) and prostaglandins ?2 (PG?2) from lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and blocked LPS-induced expression of cyclooxygenase-2. To clarify mechanistic basis for its inhibitions of NF-xB and activator protein-1 (AP-1) activations, effects of OJE on activations of NF-xB and AP-1 genes by luciferase reporter activity were examined. The LPS-stimulated activations of NF-xB and AP-1 were significantly blocked by 400 and $600 u$g/mL of OJE, implicating that OJE might regulate gene expression through more than one signaling pathway. Cytosolic degradation of I-xBa was inhibited by OJE dose-dependently, indicating that the nuclear translocation of p65 was inhibited by OJE. These findings suggest that the inhibition of LPS-stimulated COX-2 expression by OJE is due to its inhibition of NF-xB activation by blocking I-xBa degradation, which may be mechanistic basis of anti-inflammatory effects of OJE.