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논문검색

Characterization and Application of a Novel Thermostable Glucoamylase Cloned from a Hyperthermophilic Archaeon Sulfolobus tokodaii

원문정보

Njoroge, Rose Nyawira, Li, Dan, Park, Jong-Tae, Cha, Hyun-Ju, Kim, Mi-Sun, Kim, Jung-Wan, Park, Kwan-Hwa

한국식품과학회 Food Science and Biotechnology Volume 14 Number 6 2005.12 pp.860-865
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

A gene for a putative glucoamylase, stg, of a hyperthermophilic archae on Sulfolobus tokodaii was cloned and expressed in Escherichia coli. The recombinant glucoamylase (STGA) had an optimal temperature of 80℃ and was extremely thermostable with a D-value of 17 hr. The pH optimum of the enzyme was 4.5. Being different from fungal glucoamylases, STGA hydrolyzed maltotriose (G3) most efficiently. Gel permeation chromatography and sedimentation equilibrium analytical ultracentrifugation analysis showed that the enzyme existed as a dimer. STGA was stable enough to hydrolyze liquefied com starch to glucose in 4 hr at with a yield of95%. Comparison of the Kcal values for the hydrolysis and the reverse reaction at 75℃ and 90℃ indicated that glucose production by STGA was more efficient at 90℃ than 75℃. Therefore, STGA showed great potential for application to the industrial glucose production process due to its high thermostability.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

키워드

저자정보

  • Njoroge, Rose Nyawira Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University
  • Li, Dan National R&D Center for Soybean Processing, and College of Biological Science and Technology, Changchun University
  • Park, Jong-Tae Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University
  • Cha, Hyun-Ju Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University
  • Kim, Mi-Sun Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University
  • Kim, Jung-Wan Department of Biology, University of Incheon
  • Park, Kwan-Hwa Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University

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