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Purpose : This study aimed to examine the three-year (2022~2024) period in job stress among workers in a manufacturing company and to identify factors influencing these changes using a linear mixed model. Methods : The study participants were 597 workers whose job stress was assessed annually from 2022 to 2024. Job stress was measured using the Korean Occupational Stress Scale (KOSS). The data were analyzed with SPSS version 25.0, applying linear mixed modeling to account for repeated measures. Results : Job stress did not show a significant change over time. However, significant predicting factors of changes in job stress included gender, age, work experience, and employment type. Conclusion : When developing and implementing workplace interventions to manage job stress among industrial workers, demographic characteristics (i.e., gender, age) and occupational factors (i.e., work experience, employment type) identified as significant factors of job stress should carefully be considered. The study is noteworthy in that it examined longitudinal trends and influencing factors of job stress over a three-year period.