원문정보
초록
영어
Hyperglycemia, a defining characteristic of diabetes, induces oxidative stress (OS) across various physiological systems. Electrolyzed alkaline reduced water (ARW) has demonstrated in vitro antioxidant properties, owing to its bioactive properties. Therefore, we conducted this study to investigate the antioxidative and anti-diabetic potential of ARW at pH 8.5 in C57BL/6 mice. In this study, C57BL/6 mice were fed a high-fat diet for four weeks to induce insulin resistance, followed by streptozotocin (STZ) injections to induce hyperglycemia. Furthermore, mice were treated with tap water (TW)_pH 7.7, metformin, and ARW_ pH 8.5 for four weeks. Post-treatment, various parameters were evaluated, including total white blood cell (WBC) and its differential counts, OS markers such as reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxidase (GPx), and catalase (CAT). In addition, other markers such as fasting blood glucose (FBG), serum glucose, kidney marker (BUN, Creatinine), liver markers (ALT & AST), adenosine triphosphate (ATP), C-peptide, parathyroid hormone (PTH) and cytokines were also measured to determine the effects of ARW_pH 8.5. Our results showed that ARW_8.5 was effective in reducing OS markers, diabetic markers, and inflammatory markers. In addition, we found that ARW_8.5 treatment enhanced GPx and ATP activity. Overall, our findings indicate that ARW_8.5 strengthened antioxidant defense system and regulated inflammation, leading to improved cellular metabolism and altering the harmful effects of hyperglycemia in C57BL/6 mice.