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Purpose : This study aims to identify the factors related to job stress and its eight subdomains among workers in a manufacturing workplace. Methods : The study surveyed 597 workers from a manufacturing facility in P region, Gyeonggi Province, South Korea, using the Korean Occupational Stress Scale (KOSS Basic Version) from May 1 to 31, 2022. The data were analyzed using the SPSS WIN 21.0. Descriptive statistics were used to assess demographic, occupational, and healthy behavioral characteristics, along with job stress and its subdomains. Differences in job stress and subdomains based on these factors were evaluated using one-way ANOVA. Stepwise linear regression analysis identified factors related to job stress. Results : Key factors related to overall job stress included work experience, gender, and work type, accounting for 6.8% of the variance. In the subdomains, physical environment was associated with work type. In job demands, gender and work experience were identified and in job autonomy, the factors were work type and gender. Work experience was significant concerning conflicts. Also, job instability was associated with work experience and physical activity. In organizational structure, the factors were work experience and gender. For inadequate compensation, gender, work type, and work experience were significant. Furthermore, workplace culture was influenced by work experience, gender, and alcohol consumption. Conclusion : Job stress in manufacturing workers affects mental, physical, and behavioral aspects. The findings highlight the need for tailored intervention programs targeting each subdomain of job stress to address these diverse impacts.