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Objectives: This study analyzed the influence of organizational cynicism among ward sisters on their organizational citizenship behavior and how coaching leadership moderated it. Methods: Survey data from 500 ward sisters at 10 general hospitals were collected, and the data of 483 ward sisters were used. The IBM SPSS AMOS Ver. 21.0 and PROCESS macro software were used for conducting regression analysis, validity analysis, and regulation effect analysis. Results: Organizational cynicism was negatively related to organizational citizenship behavior. Coaching leadership strengthened the negative influence of organizational cynicism on organizational citizenship behavior. Conclusion: This study empirically identified that organizational cynicism is an important predisposing factor affecting the organizational citizenship behavior of ward sisters. In order for coaching leadership to exert a positive influence on organizational citizenship behaviors, addressing ward nurses’ degree of organizational cynicism and psychological status should be prioritized.