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Objectives : The purpose of this study was to examine the relationship between the health behavior and prevalence of chronic diseases among manufacturing workers. It would provide fundamental data in the development of health promotion programs for manufacturing workers. Methods : Data on 3,171 employees who underwent health check-ups by the National Health Insurance Service in L company, G City from March to December 2014 were analyzed. The statistical analysis of frequency, chi-square test, and multiple logistic regressions were performed using SPSS 18 program. Results : The results of this study show that obesity and over-weight are the health behaviors that influence the prevalence of chronic diseases in manufacturing employees. Conclusions : The implementation of public health projects to improve the voluntary participation of the employees can enhance their health, improve the productivity, and influence their quality of life positively by changing the health behaviors.