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Purpose: The purpose of this study was to examine the effect of health education based on life skill training (LST) on middle school students' health knowledge, attitude, and behavior. Methods: For this study, 961 middle school students, residing in Seoul, Korea, were selected. The students received health education one day a week for 7 weeks from September 1, 2014 to October 25, 2014 . Before and after the whole education program, the students filled out questionnaires surveying on their health knowledge, attitude, and behavior. Excluding inappropriate data, the data of 843 students was analyzed through Paired t-test. Results: After the education program based on LST, there were statistically significant increases in the scores for knowledge about drinking, body image distortion, good eating habits, right usage of smartphone, and prevention of smartphone addiction (p<.05). There were also statistically significant increases in the scores for attitude toward smoking, drug, good eating habits and prevention of smartphone addiction (p<.05). There were statistically significant increases in the scores for behavior regarding drug, good eating habits, right usage of smartphone and prevention of smartphone addiction (p<.05). Conclusion: Health education based on life skill training was especially effective in improving the subjects’ scores for health knowledge, attitude, and health behavior. Therefore, various and systemic LST educational programs should be developed and implemented and their effects should be evaluated in further studies.