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영어

Gramoxone intion supplemental agent® contains methanol, 10 % polyoxyethylene alkyl aryl ether, 20% sodium ligno sulfate as the surfactant, and various minor components. Methanol is a common ingredient in many solvents, windshield-washing solutions, duplicating fluids, and paint removers. Methanol s metabolites may cause metabolic acidosis, blindness, and death after a characteristic latent period of 6~30 hours. The surfactants commonly used in herbicide products serve several purposes, including acting as promoting uniform spread of herbicide on the leaf surface, wetting agents, and assisting the penetration of herbicide into the leaf. The surfactants is usually considered to be mainly or partly responsible for the toxic effects of various pesticides, inducing gastrointestinal tract, pulmonary, and depressive cardiac effects. We report a case of Gramoxone intion supplemental agent®. For analysis of methanol and self-poisoning polyoxyethylene alkyl aryl ether, it was used GC/FID and LC/MS, respectively. GC/FID method to measure of methanol was used by a Varian GC-3800 with headspace autosampler. For analysis of polyoxyethylene alkyl aryl ether, biological specimens were extracted directly with CHCl3. The LC system was coupled to a 3200 QTrap mass spectrometer using electrospray ionization in positive mode and multiple reaction monitoring (MRM) mode. Liquid-chromatographic analysis was performed in gradient mode on a Cadenza CD-C18 column (3 ㎛, 100 x 2 mm). The mobile phase consisted of A: 1 % aqueous acetic acid and B: acetonitrile (containing 1 % acetic acid). The flow rate was 0.3 mL/min. The methanol concentrations were 118 mg/L in peripheral blood, 120 mg/L in heart blood, 122 mg/L in gastric contents and 10 % (v/v) in Gramoxone intion supplemental agent bottle. The retention times and fragment ion ratios of surfactants selected for analysis were equal in biological specimens, compared with those of Gramoxone intion supplemental agent® bottle, which supported the deceased ingested Gramoxone intion supplemental agent. This suggests that the agrichemical poisoning should consider the toxicity of the adjuvants as well as active ingredients.