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영어

Using universal and species-specific DNA markers, the identification of plant species from rice cake pastes (0.83gram) submitted as evidences in a fraud case was handled in this work. One universal species identification marker and rice-, wheat-, mulberry-, and mugwort-specific DNA markers were used for genetic identification of plant contents from rice cake pastes in powdered form. DNAs of submitted rice cake powder were purified with QIAGEN DNeasy Plant Mini Kit and amplified with primer pairs of each genetic region. The acquired sequences of universal trnL-trnF intergenic spacer (IGS) from evidence were compared with DNA databases in National Center for Biotechnology Information (NCBI) and so they were identical to the ramie sequences. Also, we tried to identify the plant constituent parts of evidence using a presence/absence test of each species-specific marker. The rice RM171 marker and sequence characterized amplified regions (SCAR) marker of mugwort showed specific amplicons from evidence’s DNA, but waxy marker of wheat and chalcone isomerase marker of mulberry did not. According to these analysis results, the plant contents of rice cake powder were rice, ramie, and mugwort. The powder did not contain wheat and mulberry.