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The purpose of this study was to identify the effect nursing organizational culture and happiness index on turnover intention among nurses. The subjects of this study were 377 nurses who were working at 3 general hospitals in B city. The data were collected by structured questionnaire from July 1 to August 28 of 2013. The data collected were analyzed with SPSS Win 20.0 using descriptive methods, t-test, ANOVA, Pearson correlation coefficient and Stepwise multiple regression. Turnover intention were significant negative correlation for affiliative oriented organizational culture(r=-.137, p=.008), happiness index(r=-.290, p<.001). There were significant positive correlation for innovative oriented organizational culture(r=.123, p=.017), rank oriented organizational culture(r=.126, p=.015), task oriented organizational culture(r=.218, p<.001). Factors affecting for turnover intention were happiness index(β=-.297, p<.001), rank oriented organizational culture(β=.266, p<.001), nursing experience(β=.199, p=.009), affiliative oriented organizational culture(β=-.142, p=.034). The explained variances for were turnover intention among nurses 17.2%. Based on the study consider to development and education program of happiness index and affiliative oriented organizational culture for nurses in the hospital setting.