토마토 잎 초임계 추출물의 피부 색소침착 억제 효과


Inhibition Effect of Tomato Leaves Supercritical Extracts on Skin Pigmentaion

오봉윤, 이유석, 강정화, 남승희, 이선경, 안인숙, 김은희, 정경주

피인용수 : 0(자료제공 : 네이버학술정보)



In this study, we investigated inhibition effects of tomato leaves supercritical extracts on skin pigmentaion. The leaves of tomato (Lycopersicon esculentum) contains functional ingredients such as lycopene, lutein, chlorophyll, caffeic acid, and antioxidant studies prior showed a whitening effect in vitro. RT-PCR and western blotting analysis, tomato leaves supercritical extract with 0.01mg/mL concentration showed the mostly inhibited tyrosinase activity on B16F1 cell. Supercritical extract of leaves of tomato, tomato half of the colorant was prepared at a concentration of 0.5% (T-0.5%), and 1% (T-1%), formed by UVB radiation in the Hr-1 mice using a mexameter after coated four times once eight days to two days supercritical extract and measured the level of melanin. The 6 day, melanin levels were significantly reduced in the T-1% (332.14±24.91) as compared to N.cont group (428.72±48.39). The 8 day, melanin levels were significantly decreased in T1% group (261.25±16.32) and T-0.5% group (282.32±26.43). After changing anatomy of the expression level of Matrix metalloproteinase-2, 9 (MMP-2, MMP-9), were collected tissue sample application portion investigation and the expression level of MMP-2 and MMP-9 as compared with N.cont group expression levels were significantly decreased T-1% group (405.11±23.94) and T-1% group (421.54±32.81). As a result of the above, the results of animal experiments using the HR-1 mice, supercritical extract of tomato leaves, it was confirmed that there is a inhibition effect of skin pigmentaion and moisturizing and whitening effect.


 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 토마토 잎 초임계 추출 및 유효성분 확인
  2. Tyrosinase inhibition assay
  3. MTT assay에 의한 세포독성에 미치는 토마토 잎 추출물의 영향
  4. RT-PCR 분석에 의한 Tyrosinase, TRP-1, TRP-2 및MITF mRNA 발현에 미치는 토마토 잎 추출물의 영향
  5. Western blotting 분석에 의한 tyrosinase 단백질 발현에 미치는 토마토 잎 추출물의 영향
  6. HR-1 hairless mouse 사육 및 시료 처리
  7. 자외선 조사 및 시료 처리
  8. 멜라닌, 경피수분손실량 측정
  9. 결과 분석
 Ⅲ. 연구결과 및 고찰
  1. 토마토 잎 추출물의 유효성분 함량 분석
  2. 토마토 잎 추출물의 tyrosinase 활성 저해
  3. 토마토 잎 추출물의 세포독성
  4. 토마토 잎 추출물의 tyrosinase, TRP-1, TRP-2 및 MITF mRNA 발현에 미치는 영향
  5. Tyrosinase 단백질 발현에 미치는 토마토 잎 추출물의 영향
  6. 색소침착 개선 효과
  7. 표피 수분량 개선 효과
  8. MMP 발현량 변화
 Ⅳ. 결론


  • 오봉윤 BongYun Oh. 전남농업기술원 식품경영연구소
  • 이유석 You-Seok Lee. 전남농업기술원 식품경영연구소
  • 강정화 Jeong-Hwa Kang. 전남농업기술원 식품경영연구소
  • 남승희 Seung-Hee Nam. 전남농업기술원 식품경영연구소
  • 이선경 Sun-Kyung Lee. 전남농업기술원 식품경영연구소
  • 안인숙 In-Sook An. 한국피부임과학상연구소
  • 김은희 Eun Hee Kim. 경복대학교 미용예술학부
  • 정경주 Kyung-Ju Jung. 전남농업기술원 식품경영연구소


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