원문정보
Inhibition Effect of Tomato Leaves Supercritical Extracts on Skin Pigmentaion
초록
영어
In this study, we investigated inhibition effects of tomato leaves supercritical extracts on skin pigmentaion. The leaves of tomato (Lycopersicon esculentum) contains functional ingredients such as lycopene, lutein, chlorophyll, caffeic acid, and antioxidant studies prior showed a whitening effect in vitro. RT-PCR and western blotting analysis, tomato leaves supercritical extract with 0.01mg/mL concentration showed the mostly inhibited tyrosinase activity on B16F1 cell. Supercritical extract of leaves of tomato, tomato half of the colorant was prepared at a concentration of 0.5% (T-0.5%), and 1% (T-1%), formed by UVB radiation in the Hr-1 mice using a mexameter after coated four times once eight days to two days supercritical extract and measured the level of melanin. The 6 day, melanin levels were significantly reduced in the T-1% (332.14±24.91) as compared to N.cont group (428.72±48.39). The 8 day, melanin levels were significantly decreased in T1% group (261.25±16.32) and T-0.5% group (282.32±26.43). After changing anatomy of the expression level of Matrix metalloproteinase-2, 9 (MMP-2, MMP-9), were collected tissue sample application portion investigation and the expression level of MMP-2 and MMP-9 as compared with N.cont group expression levels were significantly decreased T-1% group (405.11±23.94) and T-1% group (421.54±32.81). As a result of the above, the results of animal experiments using the HR-1 mice, supercritical extract of tomato leaves, it was confirmed that there is a inhibition effect of skin pigmentaion and moisturizing and whitening effect.
목차
Ⅰ. 서론
Ⅱ. 연구방법
1. 토마토 잎 초임계 추출 및 유효성분 확인
2. Tyrosinase inhibition assay
3. MTT assay에 의한 세포독성에 미치는 토마토 잎 추출물의 영향
4. RT-PCR 분석에 의한 Tyrosinase, TRP-1, TRP-2 및MITF mRNA 발현에 미치는 토마토 잎 추출물의 영향
5. Western blotting 분석에 의한 tyrosinase 단백질 발현에 미치는 토마토 잎 추출물의 영향
6. HR-1 hairless mouse 사육 및 시료 처리
7. 자외선 조사 및 시료 처리
8. 멜라닌, 경피수분손실량 측정
9. 결과 분석
Ⅲ. 연구결과 및 고찰
1. 토마토 잎 추출물의 유효성분 함량 분석
2. 토마토 잎 추출물의 tyrosinase 활성 저해
3. 토마토 잎 추출물의 세포독성
4. 토마토 잎 추출물의 tyrosinase, TRP-1, TRP-2 및 MITF mRNA 발현에 미치는 영향
5. Tyrosinase 단백질 발현에 미치는 토마토 잎 추출물의 영향
6. 색소침착 개선 효과
7. 표피 수분량 개선 효과
8. MMP 발현량 변화
Ⅳ. 결론
참고문헌