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Exogeneous oxidative stress can cause rapid change of the hair follicles and melanocyte aging process, so it is an indicator of a programmed cell death (apoptosis) resulting in hair loss. In this study, effects of natural antioxidants from wheat sprout extract on hair growth of C57BL/6 mouse (female) were evaluated. Wheat sprout and/or wheatgrass provides chlorophyll, amino acids, minerals, vitamins and enzymes. Thus it could be used for hair-promoting ingredients. Hydrophobic layers (80% ethanol, open column) of wheat sprout (fr. 5 and fr. 8) were used, with minoxidil (3%) on five patients as a positive control. With one week adaption, hair growth of 6-week old C57BL/6 mouse (female) was observed after 4 weeks of back hair removal. Transdermal extraction and hematoxylin and eosin (H&E) staining and toluidin blue staining of skin tissue were used before observation with an optical microscope, which showed similar hair growth in the positive control minoxidil (3%) as wheat sprout (fr. 5), while slower in wheat sprout (fr. 8). The slaughtering cervical tissue at 4 weeks after the C57BL/6 mouse with wheat sprout (fr. 5) started the transition to growth. Hematoxylin & eosin stained histological analysis of wheat sprout (fr. 5) showed more follicles on the skin surface and smaller mast cells (shown with toluidin blue dye). In summary, wheat sprout extract (fr. 5) on C57BL/6 mouse in dermal application and histologic analysis promotes the hair growth, predicting an application of wheat sprout extract in a wide range of hair remedy products.