밀싹 에탄올 추출물의 C57BL/6 mouse에 대한 발모 효과


Effect of Ethanolic Extract from Wheat Sprout on Hair Growth of C57BL/6 mouse

류은미, 서관우, 기근홍, 신현재

피인용수 : 0(자료제공 : 네이버학술정보)



Exogeneous oxidative stress can cause rapid change of the hair follicles and melanocyte aging process, so it is an indicator of a programmed cell death (apoptosis) resulting in hair loss. In this study, effects of natural antioxidants from wheat sprout extract on hair growth of C57BL/6 mouse (female) were evaluated. Wheat sprout and/or wheatgrass provides chlorophyll, amino acids, minerals, vitamins and enzymes. Thus it could be used for hair-promoting ingredients. Hydrophobic layers (80% ethanol, open column) of wheat sprout (fr. 5 and fr. 8) were used, with minoxidil (3%) on five patients as a positive control. With one week adaption, hair growth of 6-week old C57BL/6 mouse (female) was observed after 4 weeks of back hair removal. Transdermal extraction and hematoxylin and eosin (H&E) staining and toluidin blue staining of skin tissue were used before observation with an optical microscope, which showed similar hair growth in the positive control minoxidil (3%) as wheat sprout (fr. 5), while slower in wheat sprout (fr. 8). The slaughtering cervical tissue at 4 weeks after the C57BL/6 mouse with wheat sprout (fr. 5) started the transition to growth. Hematoxylin & eosin stained histological analysis of wheat sprout (fr. 5) showed more follicles on the skin surface and smaller mast cells (shown with toluidin blue dye). In summary, wheat sprout extract (fr. 5) on C57BL/6 mouse in dermal application and histologic analysis promotes the hair growth, predicting an application of wheat sprout extract in a wide range of hair remedy products.


 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 연구대상
  2. 실험재료
  3. 연구방법
 Ⅲ. 연구결과 및 고찰
  1. Mouse 육안적 관찰(외형 효과)
  2. Mouse 육안적 관찰(등 안쪽 조직)
  3. Mouse 조직학적 분석(hematoxylin & eosin)
  4. Mouse 조직학적 분석(toluidine blue 염색)
 Ⅳ. 결론
 감사의 글


  • 류은미 Eun-Mi Ryu. 조선대학교 생명화학공학과
  • 서관우 Gwan-Woo Seo. 조선대학교 생명화학공학과
  • 기근홍 Keun-Hong Kee. 조선대학교 의학전문대학원 병리학교실
  • 신현재 Hyun-Jae Shin. 조선대학교 생명화학공학과


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