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The purpose of this study is to determine effects of functional property of wearing hot suit with reinforced moisture permeability on energy expenditure and exercise fatigue changes. Eleven athletes from Korean wrestling team of Y University in Kyungki-do participated in this study. All experimental procedures were conducted in a laboratory under 50-55% relative humidity and 24-25°C room temperature condition. Prior to this study experiment, VO2max of all participants was calculated for exercise intensity set-up through pre-test followed by ACSM protocol. They conducted exercise under 65% VO2max with 30 mins wearing hot suit and wearing general polyester suit without moisture permeability three times each. Weight loss, energy expenditure, blood lactate level, and subjective exercise intensity were measured and compared. There was significant difference in weight loss for wearing hot suit compared to general polyester suit (p<0.05). There was significantly higher exercise efficacy and total energy expenditure in hot suit compared to general polyester suit (p<0.05). Energy expenditure change at each exercise section was significantly higher in general polyester suit and hot suit compared to non wearing suit. Moreover, there was significantly higher energy expenditure in wearing hot suit compared to general polyester suit. Subjective exercise intensity changes showed that there was no interaction among suits and increased exercise time; however, wearing hot suit showed lower subjective exercise intensity changes at each exercise section compared to general polyester suit. Effects of wearing general polyester suit on blood cholesterol change was higher than wearing hot suit (p<0.01); however, there was no significant difference in wearing hot suit and not wearing suit. In conclusion, it can be expected to have positive effect of wearing hot suit on maximizing diaphoretic effect and decreasing psychological stability and fatigue.