원문정보
Assessment of the Changes in the Microbial Community in Alkaline Soils using Biolog Ecoplate and DGGE
초록
영어
Soil microbial community analysis of farmland soil sprayed with lye in order to use fertilizer in Nigeria was performed. As a control, two kinds of soils not sprayed with lye, located in Eungo and Lagos with general practice in agriculture was selected. Soil sprayed with lye was pH 8.25 through alkalization reaction, while the other soil samples were pH 6.22 and 5.94. Substrate utilization and species diversity index of soil sprayed with lye were low than that of the other soils with the analysis of Biolog ecoplate. As a result of principal component analysis, the relationship between three samples was low. Microbial community analysis was performed by DGGE and most of them were soil uncultured bacterium. Especially, Uncultured Acidobacteria and Uncultured Methylocystis sp., which had been isolated from the rhizosphere of soybean grown in that site were discovered in the soil sprayed with lye.
목차
1. 서론
2. 실험재료 및 방법
2.1. 토양의 시료채취와 pH 측정
2.2. Community-level physiological profiling (CLPP) 분석
2.3. Denaturing gradient gel electrophoresis (DGGE) 기법을 이용한 미생물 군집 분석
3. 결과 및 고찰
3.1. 토양의 pH
3.2. Community-level physiological profiling (CLPP) 분석
3.3. Denaturing gradient gel electrophoresis (DGGE) 기법을 이용한 미생물 군집 분석
4. 결론
사사
REFERENCES