earticle

논문검색

연구논문

Biolog Ecoplate와 DGGE 방법을 이용한 알칼리화 토양의 미생물군집 변화 평가

원문정보

Assessment of the Changes in the Microbial Community in Alkaline Soils using Biolog Ecoplate and DGGE

이은영, 홍선화

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

Soil microbial community analysis of farmland soil sprayed with lye in order to use fertilizer in Nigeria was performed. As a control, two kinds of soils not sprayed with lye, located in Eungo and Lagos with general practice in agriculture was selected. Soil sprayed with lye was pH 8.25 through alkalization reaction, while the other soil samples were pH 6.22 and 5.94. Substrate utilization and species diversity index of soil sprayed with lye were low than that of the other soils with the analysis of Biolog ecoplate. As a result of principal component analysis, the relationship between three samples was low. Microbial community analysis was performed by DGGE and most of them were soil uncultured bacterium. Especially, Uncultured Acidobacteria and Uncultured Methylocystis sp., which had been isolated from the rhizosphere of soybean grown in that site were discovered in the soil sprayed with lye.



목차

Abstract
 1. 서론
 2. 실험재료 및 방법
  2.1. 토양의 시료채취와 pH 측정
  2.2. Community-level physiological profiling (CLPP) 분석
  2.3. Denaturing gradient gel electrophoresis (DGGE) 기법을 이용한 미생물 군집 분석
 3. 결과 및 고찰
  3.1. 토양의 pH
  3.2. Community-level physiological profiling (CLPP) 분석
  3.3. Denaturing gradient gel electrophoresis (DGGE) 기법을 이용한 미생물 군집 분석
 4. 결론
 사사
 REFERENCES

저자정보

  • 이은영 Eun Young Lee. 수원대학교 환경에너지공학과
  • 홍선화 Sun Hwa Hong. 수원대학교 환경에너지공학과

참고문헌

자료제공 : 네이버학술정보

    함께 이용한 논문

      ※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

      0개의 논문이 장바구니에 담겼습니다.