원문정보
Effects of Silkworm Gland Hydrolysate on Albumin-erythropoietin Production in Transgenic Chinese Hamster Ovary Cells
초록
영어
To date, various strategies have been studied to increase specific productivity in Chinese hamster ovary (CHO) cell cultures. Also, albumin-fusion platform is being applied to other important bioactive peptides with short half-lives. Here, we investigated the effects of silkworm gland hydrolysate (SGH) on the production of albumin-erythropoietin (Alb- EPO) in transgenic CHO cells. The viable cell density of CHO cells was increased by 13% in the medium containing 1 mg/mL SGH higher than in the control medium without SGH. In addition, the production of Alb-EPO was also 1.26- fold enhanced by reducing the early apoptosis of CHO cells. In conclusion, SGH could be used as a useful supplement for the enhancement of recombinant protein production.
목차
1. 서론
2. 재료 및 방법
2.1. 세포주 및 세포배양
2.2. 누에 실샘 가수분해물의 제조
2.3. 세포 수 및 생존도 측정
2.4. 배지 내 Alb-EPO의 정량분석
2.5. 세포 주기 분석
2.6. 세포사멸 분석
2.7 통계처리
3. 결과 및 고찰
3.1. CHO 세포 증식능 및 Alb-EPO 생산성 비교
3.2. SGH 농도에 따른 세포 주기의 변화
3.3. SGH의 세포사멸 억제 및 생산량 증대 효과
3.4. 세포사멸 분석
4. 결론
감사
REFERENCES