원문정보
초록
영어
An endocrine system in microfluidics was devised by coupling together two different cell types, β-cells from the pancreas and L-cells from the intestine. L-cells secrete GLP-1 as a function of the glucose levels, thereby increasing insulin production in β-cells. The two cell lines, INS-1 cell line (L-cell) and GLUTag cell line (β-cell) were separately cultured in different compartments of 3D microfluidic cell culture system with pillar arrays [1]. Both cell lines formed aggregates and exhibited 3D cell morphology and showed a good cell viability (> 95%) after 3 days of culture. In response to the glucose stimuli, INS-1 and GLUTag cells produce higher amounts of insulin and GLP-1, respectively, compared to those in a 2D cell culture. Furthermore, insulin production was further increased by connecting the two cell culture compartments. We have demonstrated that a microfluidic-based endocrine system is suitable for observing dynamic change of endocrine hormones (insulin and GLP-1) upon glucose intake. This system can be potentially used to screen drug candidates against diabetes.