원문정보
초록
영어
To develop a new cultivar of King oyster mushroom(Pleurotus eryngii), G09-21 as parental strain was selected by the method of Di-mon crossing between monokaryotic strains derived from ASI 2824(Keunneutari No.2) and dikaryotic strain ASI 2887(Aeryni 3). The Pe21-51(G09-21-10 x 2844-11) was shown the best cultural characteristics, selected to be a new cultivar and named as ‘Song-A’. The ‘Song-A’ was formed incompatibility line distinctly in the confrontation growth of parental strains Keunneutari No.2, Aeryni 3 and ASI 2844. The optimum temperature for mycelial growth, fruiting body development and pH arrange were 25~30℃, 14~16℃ and pH5~8, respectively. Fruiting body production per bottle was about 94.7±29.5 g which is almost 106% quantity compared to that of other cultuvar Keunneutari No.2. And also the stip is thick and long but the number of available stipe is few. Analysis of the genetic characteristics of the new cultivar ‘Song-A’ showed a different DNA profile as that of the control strains, Keunneutari No.2, Aeryni 3 and ASI 2844, when RAPD(Random Amplified Polymorphic DNA) primers URP4 and 7 were used. This new cultivar ‘Song-A’ of Pleurotus eryngii is characterized by a small number of primordia formation and the stip is thick and long. Therefore, we expect that this new strain will save of labor and cost by without culling work.
목차
서론
재료 및 방법
시험균주 및 배양
단핵균주 분리 및 교잡계통 육성
DNA 다형성 분석
교잡계통 선발을 위한 자실체 특성 분석
결과 및 고찰
육성경위
'송아'의 균사 배양적 특성 및 대선형성 유무
'송아'의 DNA다형성 검정
'송아'의 자실체 특성
적요
참고문헌
