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총설

ITS 부위의PCR-RFLP 및 STS 마커를 이용한 차가버섯의 종 및 계통간 유연관계 분석

원문정보

Identification and Phylogenetic Relationships of Inonotus obliquus Strains by PCR-RFLP of ITS sequences and STS markers

신평균, 공원식, 유영복, 이금희, 오세종, 최만수

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

Because the import of Inonotus obliquus have been rapidly increased in Korea, we developed the Inonotus species-specific marker by using various sequences including ITS sequences, PCR-RFLP and STS primers and used this marker to determine both genetic relatedness and strains discrimination of Inonotus spp. Total 17 different Inonotus spp. were examined by using ITS sequences and classified into 2 different groups. One strain, ASI74008 isolated from Kamchaka island of Siberia, showed the high sequence identity (98%) at the nucleotide level to the other I. obliquus DSM strain, indicating the ASI74008 belong to I . obliquus species. Comparison of banding patterns after restriction enzyme digestions with PCR amplicons of ITS region revealed some variations depending on the species and strains. However, PCR products amplified with STS primer showed species specific patterns.Therefore, use of both STS primers and PCR-RFLP could help for better strain identification.

목차

ABSTRACT
 서론
 재료 및 방법
  균주분리 및 배양
  DNA 추출 및 PCR 증폭
  염기서열 및 유연관계 분석
 결과 및 고찰
 적요
 참고문헌

저자정보

  • 신평균 Pyung-Gyun Shin. 농촌진흥청 국립원예특작과학원 버섯과
  • 공원식 Won-Sik Kong. 농촌진흥청 국립원예특작과학원 버섯과
  • 유영복 Young-Bok Yoo. 농촌진흥청 국립원예특작과학원 버섯과
  • 이금희 Keum-Hee Lee. 국립식물검역원 중부격리재배관리소
  • 오세종 Se-Jong Oh. 국립식량과학원 맥류사료작물과
  • 최만수 Man-Soo Choi. 전작과

참고문헌

자료제공 : 네이버학술정보

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