원문정보
A Research for the Development of Beauty Functional Materials of Ethanol Extract of Agrimonia pilosa
초록
영어
The development of a variety of cosmetic and beauty is being promoted as our understanding of the dermatology in differentiation of skin cells, the identification of aging, and the development of artificial skin cells, has advanced with the brilliant development of biotechnology. In this study, we examined the function of physiological act from outposts of Agrimonia pilosa extracted by ethanol. Electron donating ability was increased in proportion to the concentration of the antioxidant activity. When we measured polyphenols and flavonoids contents of Agrimonia pilosa, the result was respectively 105.1±1.5, 32.8±2.7 mg/ml. Cytotoxicity was not found under 50 μg/ml in concentration. The protective effect about damaged HaCaT cells by hydrogen peroxide increased in a dose dependent. And measuring anti-inflammatory activity , we could also know that NO production was significantly reduced within the concentration range. Thus, ethanol extract of Agrimonia pilosa was identified as a beauty functional material.
목차
Ⅰ. 서론
Ⅱ. 연구재료 및 방법
1. 재료 및 시료의 추출
2. 총 폴리페놀 함량 측정
3. 총 플라보노이드 함량 측정
4. 전자공여능 측정
5. 세포 배양
6. 세포생존율 측정
7. H2O2에 의한 세포 손상 보호 효과 측정
8. 항염증 활성 측정
9. 통계분석
Ⅲ. 연구결과 및 고찰
1. 수율 및 총 폴리페놀 및 플라보노이드 함량
2. 전자공여능
3. 세포 생존율
4. H2O2에 의한 세포 손상 보호 효과
5. 항염증 효과
Ⅴ. 결론
참고문헌