진피 플라보노이드 생물전환 균주 분리 및 췌장 베타세포에 대한 산화적 손상 억제 효과


Isolation of Citrus Peel Flavonoid Bioconversion Microorganism and Inhibitory Effect on the Oxidative Damage in Pancreatic Beta Cells

박치덕, 정희경, 박창호, 정유석, 홍주헌, 고희선, 강동희, 김현수

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In this study, the optimum conditions of fermentation were determined by isolating the microorganisms with the ability to bioconvert the Citrus peel flavonoid, and the effect of the fermented Citrus peel extract which was bioconverted on the oxidative damage of HIT-T15 cell was investigated. The Aureobasidium pullulans Y-12 was isolated and identified with the strains having bioconversion activity. The fermentation conditions for bioconversion activity were confirmed to be optimal when culturing for three days at 25℃, 150 rpm in a culture medium containing 5% Citrus peel power and 0.8% casitone. As a result of bioconversion, 32.8 mg/g and 21.5 mg/g of naringenin and hesperetin, which were aglycone flavones, were produced respectively. Also in the flavonoid content, it was confirmed that FCP produced 154.8 mg/g while CP produced 33.7 mg/g, thus producing more by approximately 4.6 times. As a result of treating FCP and CP after inducing the oxidative damage for HIT-T15 cell by treating the deoxy-D-ribose with IC50 (38 mM) concentration, the surviving rate was recovered to 90% for FCP treatments in the 0.01 mg/mL concentration and for CP treatments in the 0.025 mg/mL concentration. Also in the insulin secretion rate, FCP treatments increased by 206% and CP treatments by 132% when treated in the 0.1 mg/mL concentration. As the bioconverted FCP can inhibit the oxidative damage of HIT-T15 cell in the low concentration, it was considered its usability as the functional material for prevention of the type 2 diabetes.


 1. 서론
 2. 재료 및 방법
  2.1. 실험재료
  2.2. 생물전환 균주 분리 및 동정
  2.3. β-glucosidase 활성
  2.4. 시료 제조 및 플라보노이드 함량
  2.5. 생물전환을 위한 발효조건
  2.6. 세포배양 및 독성
  2.7. 산화적 손상에 대한 세포 생존율 및 인슐린 분비능
 3. 결과 및 고찰
  3.1. 생물전환 균주의 분리
  3.2. 생물전환 전환물 확인
  3.3. 분리균주 동정
  3.4. 진피 발효의 최적 조건
  3.5. HIT-T15의 세포 독성
  3.6. 세포 회복능
  3.7. 인슐린 분비능
 4. 결론


  • 박치덕 Chi-Deok Park. 대구테크노파크 바이오헬스융합센터
  • 정희경 Hee-Kyung Jung. 대구테크노파크 바이오헬스융합센터
  • 박창호 Chang-Ho Park. 대구테크노파크 바이오헬스융합센터
  • 정유석 Yoo-Seok Jung. 대구테크노파크 바이오헬스융합센터
  • 홍주헌 Joo-Heon Hong. 대구가톨릭대학교 외식식품산업학부
  • 고희선 Hee-Sun Ko. 계명대학교 미생물학과
  • 강동희 Dong-Hee Kang. 계명대학교 미생물학과
  • 김현수 Hyun-Soo Kim. 계명대학교 미생물학과


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