원문정보
Anticariogenic Activities of Lactobacillus sakei K-7 Isolated from Kimchi
초록
영어
The occurrence of dental caries is mainly associated with oral pathogens, especially cariogenic Streptococcus mutans. The aim of this study was to isolate and characterize lactic acid bacterium showing inhibitory activity against cariogenic Streptococcus mutans. As results, an isolate with strong inhibitory activity was obtained from Kimchi and it was identified as Lactobacillus sakei by API and 16S rRNA gene analyses. This strain secreted an inhibitory compound in cell growth medium and the activity of the compound was completely disappeared by proteinase K revealing the fact that the compound is proteinous substance, bacteriocin. Optimal culture condition for bacteriocin production by Lb. sakei K-7 was at pH 7.5 and 37℃ for 18 h. Oral administration of this isolate may give anticariogenic and probiotic effects on hosts.
목차
1. 서론
2. 재료 및 방법
2.1. 재료
2.2. 사용 균주
2.3. 항충치 활성 유산균의 분리
2.4. 항충치능 물질 제조
2.5. 박테리오신 활성 확인
2.6. 항충치 활성 유산균 동정
2.7. 항충치 물질의 이화학적인 특성 조사
2.8. 배양 시간에 따른 항충치물질의 생산
3. 결과 및 고찰
3.1. 항충치 활성 유산균의 분리 및 동정
3.2. 생성 박테리오신의 이화학적 특성
3.3 발효기간별 박테리오신 생산
4. 결론
References