원문정보
1,2-Propanediol Production by Using Saccharomyces cerevisiae M3G3
초록
영어
1,2-propanediol (1,2-PD) is a commodity chemical that is currently produced from petrochemical derivatives. Saccharomyces cerevisiae is well characterized and a successful industrial microorganism to enable the improvement of the 1,2-propanediol production by metabolic engineering. A recombinant S. cerevisiae M3G3 was used to produce 1,2-propanediol. S. cerevisiae M3G3 is the diploid strain that contains 3 copies of mgs (methylglyoxal synthase) and gldA (glycerol dehydrogenase). S. cerevisiae M3G3 was cultivated at various culture conditions by changing culture temperature, glucose concentration, and inducer concentration. Also the effect of induction time was studied to optimize the production of 1,2-propanediol. Batch and fed-batch cultivation of S. cerevisiae M3G3 was performed by using a 5 L jar fermenter. The highest concentration of 1,2-propanediol in batch cultivation was 0.86 g/L and it was further improved to 1.33 g/L in fed-batch cultivation.
목차
1. 서론
2. 재료 및 방법
2.1. 균주
2.2. 배지
2.3. 배양 온도의 영향
2.4. 글루코오스 농도의 영향
2.5. 유도물질 농도의 영향
2.6. 유도 시기의 영향
2.7. 배양
2.8. 분석
3. 결과 및 고찰
3.1. 배양 온도의 영향
3.2. 글루코오스 농도의 영향
3.3. 유도물질 농도의 영향
3.4. 유도시기의 영향
3.5. 회분식 배양과 유가식 배양에 의한 1,2-propanediol 생산
4. 결론
감사
References