원문정보
Optimization of Medium and Fermentation Conditions for Mass Production of Bacillus licheniformis SCD121067 by Statistical Experimental Design
초록
영어
In this work, mass production of Bacillus licheniformis SCD121067 through medium optimization by statistical experimental method was studied. First, galactose, yeast extract and potassium phosphate dibasic were selected as carbon, nitrogen and phosphate sources for mass production of B. licheniformis SCD121067 by using one factor at a time method. Second, according to the result of Plackett-Burman experimental design, key factors was yeast extract and K2HPO. Finally, the response surface methodology was performed to obtain the optimum concentrations of two selected variables. The optimized medium composition consisted of 20 g/L galactose, 36 g/L yeast extract, 0.41 g/L K2HPO4, 0.25 g/L Na2CO3, 0.4g/L MgSO4 and 0.01g/L CaCl2. Dry cell weight (15.4 g/L) by optimum production medium were increased 10 times, as compared to that determined with basic production medium (1.5 g/L). Fermentation conditions were examined for the mass production of B. licheniformis. The effect of temperature, agitation speed, pH and aeration rate on the mass production of B. licheniformis were also studied in a batch fermenter which was carried out in a 2.5 L bioreactor with a working volume of 1.5 L containing optimized production medium. As a result, dry cell weight of batch culture was 30.7 g/L at 42℃, 300 rpm, pH 8.0 and 2 vvm.
목차
서론
재료 및 방법
사용균주 및 균주보관
배지 및 배양 조건
최적 배지성분 결정과 통계적 배지농도 최적화
발효조건 최적화
분석방법
건조균체량 (Dry cell weight, D.C.W)
Protease 측정
Bacillus cereus 항균활성
Galactose 분석
결과 및 고찰
Bacillus cereus 항균활성
One factor at a time
Plackett-Burman design을 통한 중요인자 선정
Full factorial design을 통한 B. licheniformis SCD121067 생산 배지 영향
Steepest ascent method을 통한 최적점으로의 최단거리 확립
반응표면분석법 (response surface method)을 통한B. licheniformis SCD121067 배지 최적화
발효조건 최적화와 회분식 배양
최적배지와 선택된 발효 조건을 이용한 반복회분배양
결론
REFERENCES