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논문검색

화학/분석

칼슘함량이 강화된 새송이 버섯의 프로테옴 분석

원문정보

Proteomic Characteristics of Calcium Enriched King Oyster Mushroom (Pleurotus eryngii)

배희선, 김대현, 최웅규

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

This study was conducted to identify the differences in proteomic characteristics between Ca-enriched king oyster mushrooms and general king oyster mushrooms. A combined high-throughput proteomic approach was employed to determine the expression profiles and identity of proteins using 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. The overall distribution patterns of the proteins were quite similar, but many of the protein spot intensities varied. A total of 10 proteins, representing a significant difference in the quantities of protein betweenthe two types of mushrooms, were successfully identified. Among these proteins, eight kinds were increased in the Ca-enriched king oyster mushrooms and two kinds were decreased. This study showed that proteomic analysis can help define specific changes in protein level and composition, which can occur in mushrooms where Ca content may or may not be enriched.

목차

Abstract
 서론
 재료 및 방법
  칼슘강화 새송이버섯 배양 및 칼슘함량 측정
  단백질 분리
  이차원 전기영동
  이미지 분석
  단백질 절편
  단백질 동정
  Database search
 결과 및 고찰
  새송이버섯 자실체의 칼슘함량
  이차원 전기영동 패턴
  발현 차이의 단백질 확인
  동정된 단백질의 분류
 요약
 문헌

저자정보

  • 배희선 Hee-Sun Bae. (재)포항테크노파크 바이오정보지원센터
  • 김대현 Hee-Sun Bae. 울산과학기술대학교 나노생명화학공학부
  • 최웅규 Ung-Kyu Choi. 울산과학기술대학교 나노생명화학공학부

참고문헌

자료제공 : 네이버학술정보

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