원문정보
Polysaccharide Characteristics from Hot Water Extract of Aloe saponaria Callus
초록
영어
The callus formation from inferior leaf of Aloe saponaria was induced in M & S medium supplemented with 10-30 μM NAA (α-naphthalene acetic acid) and 3-7 μM kinetin under incubation in the dark at 25℃ for 6 weeks. The hot water extract (100℃, 24 hrs) from cultured callus was obtained and the components analysis for the extract were examined to determine the callus can synthesized the bioactive component such as Aloe polysaccharide. The freeze dried extract contained the sugar of 53.2%, protein of 7.3%, ash of 18.5% and water of 21% (w/w). Two fractions (Fr-I and Fr-II) were obtained by Sepharose CL-4B gel permeation chromatography and Fr-I, major fraction was further purified with dialysis. From sugar analysis by TLC and GC, the purified Fr-I fraction consisted of glucose (77.6%), galactose (17.7%), mannose (4.7%, w/w) and uronic acid (trace). The molecular weight of purified Fr-I fraction determined by GPC was about 110 kDa.
목차
서론
재료 및 방법
재료
시료 추출 및 분리, 정제
일반성분 분석
분자량 측정
구성당의 분석
결과 및 고찰
Callus 열수 추출물의 일반성분
Gel chromatography에 의한 분획 및 분자량
다당 분획의 구성 당과 조성
결론
REFERENCES