원문정보
초록
영어
Hot water extracts of Camellia japonica flower buds were found to have the higher 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity than the other solvent extracts. Five phenolic compounds were isolated and purified fromthe ethyl acetate soluble-neutral fraction of hot water extracts by Sephadex LH-20 column chromatography and octadecylsilane-high performance liquid chromatography using the guided assay of DPPH radical scavenging. Based on massspectrometer and nuclear magnetic resonance, the isolated compounds were identified as p-hydroxybenzaldehyde (1), vanillin(2), dehydroxysynapyl alcohol (3), 7S,7'S,8R,8'R-icariol A2 (4), and (-)-epicatechin (5). Four compounds (1-4) except for 5were newly identified in this plant. Their DPPH radical scavenging activities as 50% scavenging concentration decreased inorder to 5 (20µM)>α-tocopherol (29µM)>4 (67µM)>3 (72µM)>1=2 (>250µM). These results indicate that theantioxidant effect of the hot water extract of C. japonica flower buds may partially due to 5 isolated phenolic compounds.
목차
Introduction
Materials and Methods
Results and Discussion
References
