earticle

영어

Avermectins produced by Streptomyces avermitilis is a secondary metabolite with powerful anthelmintic and insecticidal activities. Avermectin is produced into eight derivatives. Among those derivatives avermectin B1a is known to be the most effective. S. avermitilis AJ-7, high avermectin B1a producing strain which was developed through random mutation with NTG, was used throughout this research. To increase avermectin B1a production, various materials related to B1a biosynthesis (biotin, L-isoleucine, S-adenosylmethionin) were added to the main culture medium at 3.0 μg/ml, 0.5 g/L, 0.1 mM respectively. And to optimize cultivation condition, research were performed on diverse agitation speeds and aeration condition. The average result obtained from this experiments, show that avermectin B1a was produced about 372.5 mg/L at 700 rpm, 0.8 vvm conditions. Results obtained from this research to increase avermectin B1a production with addition of various materials made contribution to lab scale medium optimization and batch culture using bioreactor.