earticle

영어

The uncharacterized abf gene encoding α-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus DSM 8903, belonging to family 51 of the glycoside hydrolases, was cloned and expressed in Escherichia coli. The maximal activity of the enzyme was observed at pH 5.5 and 80°C. The half-lives of the enzyme at 65, 70, 75, 80, and 85°C were 419, 141, 58, 0.8, and 0.1 h, respectively. The enzyme showed activity for pNP-α-L-arabinofuranoside and pNP-α-Larabinopyranoside among pNP-glycosides. The enzyme released Larabinose from arabinan, debranched arabinan, and arabinoxyran. However, endoarabinanase activity was not detected. Thus, α-Larabinofuranosidase from C. saccharolyticus DSM 8903 was suggested as an exo-acting enzyme.