earticle

영어

Celandine (Chelidonium majus, family Papaveraceae) is an herb used extensively in traditional Korean medicine.To investigate its antioxidant and antiproliferative activities, the methanolic extract of celandine was introduced. Theantioxidant properties of the extract were tested using various in vitro systems, including hydroxyl radical scavenging assay,DNA damage protection assay, 1,1-diphenyll-2-2-pricylhydrazyl (DPPH) free radical scavenging activity, metal chelatingactivity, and reducing power assay. The extract exhibited stronger antioxidant activity (IC50=7.92 µg/mL) against hydroxylradicals in the Fenton system than butylated hydroxyanisole (IC50=51.46 µg/mL) and α-tocopherol (IC50=67.48µg/mL).Likewise, damage to the plasmid pBR 322 induced by hydroxyl radicals was found to be protected by the extract at aconcentration of 400µg/mL. Cellular proliferation and the induction of apoptosis were also examined by a cellularproliferation assay, flow cytometry, and mRNA expression analysis. Taken together, the extract significantly inhibited thegrowth of HT-29 cells in a concentration- and time-dependent manner, and gradually increased both the proportion ofapoptotic cells and the expression of caspase-3. Overall, our research suggests that celandine possesses antioxidant andantiproliferative properties.