earticle

영어

Antioxidant activities of the extract of red seaweed, Polysiphonia morrowii, were evaluated using several in vitroassay systems. Activity-guided fractionation revealed that the 90% MeOH fraction of the P. morrowii extract exhibited thehighest antioxidant activity, and that this fraction had a high total phenolic content (135.7±5.0mg gallic acid/g extract).Therefore, the antioxidant activities of the 90% MeOH fraction against 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxylradical, reducing power, ferrous chelating, and hydrogen peroxide were investigated. The results revealed that the antioxidantactivities of the 90% MeOH fraction were similar and/or superior to that of commercial antioxidants such as butylatedhydroxyanisole (BHA) and butylated hydroxytoluene (BHT). In addition, the ability of the 90% MeOH fraction to inhibitoxidative damage to DNA was assessed by measuring the conversion of the supercoiled pBR322 plasmid DNA to the opencircular form. The 90% MeOH fraction was found to significantly protect this hydroxyl radical-induced DNA damage in adose-dependent manner. Taken together, these findings suggest that the 90% MeOH fraction of P. morrowii extract and/or itsconstituents has the potential for use as a new bioresource of antioxidants.