원문정보
The Study on the Effective Expression Strategy for Recombinant Protein Production with Pichia pastoris and Hansenula polymorpha
초록
영어
As host for the production of eucaryotic heterologous proteins, methylotrophic yeast Pichia pastoris and Hansenula polymorpha are the most highly developed of a small group of alternative yeast species chosen for their perceived advantages. This paper describes the method to enhance the recombinant protein productivity with P. pastoris and H. Plymorpha. In these experiments, the effects of methanol induction timing, induction method, pH, culture temperature and kinds of nitrogen sources on foreign protein production were tested with P. pastoris and compared with H. polymorpha.. In addition, optimum methanol concentration as inducer and the effects of carbon sources on AOX1 or MOX promoter repression and secretion efficiency were also studied in both cases.
목차
재료 및 실험방법
사용균주
배지 및 배양방법
분석방법
결과 및 고찰
메탄올 induction 시기가 발현에 미치는 영향
Inducer로서 메탄을 농도가 발현에 미치는 영향
알부민발현에 미치는 pH의 영향
배양온도가 알부민 발현에 미치는 영향
알부민 발현에 미치는 질소원의 영향
A0X1 과 MOX 프로모터 repression애 미치는 탄소원의영향
발현된 알부민의 분비효율 비교
요약
감사
참고문헌