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논문검색

Chitin의 효소적 가수분해 특성에 대한 속도론적 연구

원문정보

Kinetics on the Specificity of Enzymatic Hydrolysis of Chitin

이은영, 김광

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

Hydrolysis and adsorption reversibility experiments were run for initial enzyme activity of 4.48, 9.65, 11.19 and 17.14U/mL at a temperature 30℃. The chitin particle size corresponded to a mean particle diameter of 0.127mm, and the initial concentration of chitin was 10mg/mL. After approximately 2hrs, the enzyme activity remained constant in a speudo-steady state. The amounts in the bulk [E] and the amounts of enzyme adsorbed on the chitin surface [E] are plotted on Lineweaver-Burk plot to yield a linear relationship with a correlation coefficient of 0.99, a slope of 2.79cm and an intercept of 0.08cm2/U. From this parameters, the values of [ET] and KE were calculated to be 12.5U/cm2 and 34.88U/mL. respectively, Adsorption isotherm of the enzyme on the particles showed a well developed plateau of 1.3510, 4.7210, 4.4210, 8.5810U/cm2 at 30℃. To determine the specificity of chitinase for crystalline chitin, the free energy of adsorption was measured, and its was determined as about -14.62~-18.8kJ/mol.

목차

ABSTRACT
 서론
 재료 및 방법
  균주 및 배지
  효소 생성
  기질제조
  가수분해 반응
  흡착가역성
  분석
 결과 및 고찰
  효소의 확산- Interface mass transfer rate
  효소의 흡착
  효소-생성물 이동
  효소반응 속도론
  흡착자유에너지
 요약
 인용부호
 첨자
 참고문헌

저자정보

  • 이은영 Eun-Young Lee. 동아대학교 화학공학과
  • 김광 Kwang Kim. 동아대학교 화학공학과

참고문헌

자료제공 : 네이버학술정보

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