원문정보
Investigation of Brassinosteroid Substances in Undmia pinnatifida
초록
영어
In order to examine the presence of brassinosteroid substance in sea mustard(Undaria pinnatifida), leaves of sea mustard were extracted with MeOH. The extract was purified by slovent fractionation, counter current distribution, silica gel adsorption chromatography, charcoal adsorption chromatography, Bondesil chromatography, and reverse phase HPLC, successively. The activity was monitored by the rice inclination test and its prescence could be confirmed in each purification step. Although sea mustard contained a less amount of the active substance than the vegetative tissue of higher plants, brassinosteroid was clearly present endogenously in sea mustard. We acknowledge that our work is probably the first publication reporting the presence of brassinosteroid in marine algae plants.
한국어
미역엽상부의 MeOH추출물에 포함되어 있는 brassinosteroid활성물질을 검색하기 위하여 용매분획, CCD, silica gel흡착 chromatography, Sephadex LH-20 chromatography, charcoal 흡착 chromatography, Bondesil chromatography, 그리고 reverse phase의 HPLC등의 수법을 이용하여 분리 정제하고 각 단계에서 생물검정법으로 활성을 검정한 결과, 해조류인 미역에도 brassinosteroid활성물질의 존재가 인정되었는데, 그 함량은 타 식물의 영양조직에 비교하여 낮은 수준이었다.
목차
서론
실험재료 및 방법
실험재료
추출 및 용매분획
Counter Current Distribution(CCD)
Silica gel 흡착 chromatography
Sephadex LH-20 chromatography
Charcoal 흡착 chromatography
Bondesil chromatography
HPLC
Brassinosteroid의 활성검정
결과 및 고찰
용매분획과 활성물질의 확인
CCD
Silica gel 흡착 chromatography
Sephadex LH-20 chromatography
Charcoal 흡착 chromatography
Sephadex LH-20 chromatography
Bondesil chromatography
Preparative-HPLC
ODS column을 사용한 HPLC
고찰
요약
사사
참고문헌