원문정보
Growth Characteristics and Optimal Culture Conditions of PVA-Degrading Strains
초록
영어
PVA degrading bacteria were isolated from water system, and identified as Pseudomonas cepacia and Pseudmonas pseudomallei, which were named as Pseudomonas sp. G5Y and Pseudomonas sp. PW. It was found out that those two kinds of bacteria have a symbiotic relationship to degrade PVA. For the mixed culture of these bacteria, the optimal conditions of pH, temperature, nitrogen source, and polymerization degree of PVA were found to be 7.5, 35℃, ammonium sulfate, and 500, respectively. Also, the growth of these bacteria was promoted by trace elements such as vitamin B1, B12, pyridoxine, and p-aminobenzoate, respectively. The specific growth rate of mixed bacteria was inhibited when the concentration of PVA was more than 20g/l. The substrate inhibition kinetics of the mixed culture was {{{{= { 0.065 S} over {2.56+S+( { S}^{2 }/156) } { hr}^{-1 } }}}}
목차
서론
재료 및 방법
PVA 분해균주의 분리 및 동정
순수배양 과 혼합배양에 의한 성장특성
PVA 중합도에 따른 영향
온도 및 pH의 영향
질소원 및 미량원소의 영향
결과 및 고찰
분리 및 동정
순수배양과 혼합배양에 의한 성장특성
중합도의 영향
온도 및 pH의 영향
질소원 및 미량원소의 영향
기질저해 속도식
요약
감사
참고문헌