원문정보
Directed Evolution in Protein Functionality Improvement
초록
영어
The dynamic evolution process has resulted in the myriad shapes, functions, and systems evident in every living organism. For centuries, people have been harnessing the power of evolution to produce new varieties of plants and animals, such as producing tomatoes from berries and Chihuahuas from wolves. Now scientists are using it to produce better molecules, ranging from drugs to industrial chemicals, and doing it in days or weeks rather than eons. The
ingenious process, which creates genetic diversity and selects those with desired features in the laboratory, is called directed evolution or test tube evolution. In this paper, concepts of directed molecular evolution and some examples will be discussed.
목차
서론
방향적 진화 (Directed evolution) 의 기본 개념
A. Chemical mutagenesis
B. Error-prone PCR (Mutagenic PCR)
C. Mutagenic PCR with Random oligonucleotides
D. Saturation mutagenesis
E. Cassette mutagenesis
F. Incremental truncation
G. Homologous recombination
H. Bacterial mutator strain
I. Staggered Extension Process
J. DNA shuffling
Gene Expression System
변이주의 screening
효소의 방향적 진화와 관련된 최근의 연구
향후 연구 방향
REFERENCES